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Vectra Laboratories epn tissue scanned on vectra 3.0 imager
Epn Tissue Scanned On Vectra 3.0 Imager, supplied by Vectra Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Figure 3. Inflammation within the adipose tissue. mRNA was isolated from gWAT homogenates from mice of both genotypes on both diets. After cDNA preparation, real-time PCR was used to measure message for the indicated proteins A. Inflammatory adipocytokine F4/80 (Emr1). B. Pro-inflammatory M1 macrophage Mcp1 (Ccl2). C. Representative crown-like structures shown by <t>brightfield</t> H&E in gWAT from high fat-fed mice (black arrowheads). D. Pro- inflammatory Il-6 transcript. E. Pro-inflammatory TnfĮ transcript. F. M2 macrophage marker Arg1. G. M2 macrophage marker Tgf-ȕ1.
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Vectra Laboratories vectra image scanning
Figure 3. Inflammation within the adipose tissue. mRNA was isolated from gWAT homogenates from mice of both genotypes on both diets. After cDNA preparation, real-time PCR was used to measure message for the indicated proteins A. Inflammatory adipocytokine F4/80 (Emr1). B. Pro-inflammatory M1 macrophage Mcp1 (Ccl2). C. Representative crown-like structures shown by <t>brightfield</t> H&E in gWAT from high fat-fed mice (black arrowheads). D. Pro- inflammatory Il-6 transcript. E. Pro-inflammatory TnfĮ transcript. F. M2 macrophage marker Arg1. G. M2 macrophage marker Tgf-ȕ1.
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Figure 3. Inflammation within the adipose tissue. mRNA was isolated from gWAT homogenates from mice of both genotypes on both diets. After cDNA preparation, real-time PCR was used to measure message for the indicated proteins A. Inflammatory adipocytokine F4/80 (Emr1). B. Pro-inflammatory M1 macrophage Mcp1 (Ccl2). C. Representative crown-like structures shown by brightfield H&E in gWAT from high fat-fed mice (black arrowheads). D. Pro- inflammatory Il-6 transcript. E. Pro-inflammatory TnfĮ transcript. F. M2 macrophage marker Arg1. G. M2 macrophage marker Tgf-ȕ1.

Journal: Journal of Lipid Research

Article Title: Depletion of adipocyte sphingosine kinase 1 leads to cell hypertrophy, impaired lipolysis, and nonalcoholic fatty liver disease

doi: 10.1194/jlr.ra120000875

Figure Lengend Snippet: Figure 3. Inflammation within the adipose tissue. mRNA was isolated from gWAT homogenates from mice of both genotypes on both diets. After cDNA preparation, real-time PCR was used to measure message for the indicated proteins A. Inflammatory adipocytokine F4/80 (Emr1). B. Pro-inflammatory M1 macrophage Mcp1 (Ccl2). C. Representative crown-like structures shown by brightfield H&E in gWAT from high fat-fed mice (black arrowheads). D. Pro- inflammatory Il-6 transcript. E. Pro-inflammatory TnfĮ transcript. F. M2 macrophage marker Arg1. G. M2 macrophage marker Tgf-ȕ1.

Article Snippet: In addition, imaging services were provided by the VCU Cancer Mouse Models Core using a brightfield whole-slide scanning Vectra Polaris (PerkinElmer) automated imaging system of H&E-stained gWAT sections for assessment of crown-like structures using PhenoChart software.

Techniques: Isolation, Real-time Polymerase Chain Reaction, Marker