Journal: Journal of Lipid Research
Article Title: Depletion of adipocyte sphingosine kinase 1 leads to cell hypertrophy, impaired lipolysis, and nonalcoholic fatty liver disease
doi: 10.1194/jlr.ra120000875
Figure Lengend Snippet: Figure 3. Inflammation within the adipose tissue. mRNA was isolated from gWAT homogenates from mice of both genotypes on both diets. After cDNA preparation, real-time PCR was used to measure message for the indicated proteins A. Inflammatory adipocytokine F4/80 (Emr1). B. Pro-inflammatory M1 macrophage Mcp1 (Ccl2). C. Representative crown-like structures shown by brightfield H&E in gWAT from high fat-fed mice (black arrowheads). D. Pro- inflammatory Il-6 transcript. E. Pro-inflammatory TnfĮ transcript. F. M2 macrophage marker Arg1. G. M2 macrophage marker Tgf-ȕ1.
Article Snippet: In addition, imaging services were provided by the VCU Cancer Mouse Models Core using a brightfield whole-slide scanning Vectra Polaris (PerkinElmer) automated imaging system of H&E-stained gWAT sections for assessment of crown-like structures using PhenoChart software.
Techniques: Isolation, Real-time Polymerase Chain Reaction, Marker